FastQC ReportTue 25 Feb 2014
TestSample_1_1M.fastq
Summary
![[PASS]](Icons/tick.png)
Basic Statistics- Per base sequence quality
- Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content- Per base GC content
![[WARNING]](Icons/warning.png)
Per sequence GC content- Per base N content
- Sequence Length Distribution
- Sequence Duplication Levels
- Overrepresented sequences
- Kmer Content
Basic Statistics
| Measure | Value |
|---|---|
| Filename | TestSample_1_1M.fastq |
| File type | Conventional base calls |
| Total Sequences | 250000 |
| Sequence length | 51 |
| %GC | 53 |
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAG | 348 | 0.1392 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AGAAG | 33910 | 3.2366197 | 6.344444 | 3 |
| CTTCT | 29770 | 3.0026276 | 5.5675707 | 12 |
| CTCCA | 32010 | 2.7747195 | 6.0011115 | 6 |
| AAAAA | 20545 | 2.7685428 | 8.39936 | 2 |
| CAGCT | 31870 | 2.6950667 | 5.5626955 | 3 |
| AAGAA | 21970 | 2.4916358 | 7.2515545 | 2 |
| TGGAG | 29140 | 2.3452282 | 5.1090064 | 7 |
| GAGAA | 23640 | 2.2563758 | 5.7297573 | 2 |
| TCCAG | 26230 | 2.2181237 | 6.651895 | 7 |
| GAAGA | 22490 | 2.1466112 | 5.137024 | 2 |
| CAGGA | 25730 | 2.1186488 | 5.4164786 | 1 |
| GGAAG | 25155 | 2.0206802 | 5.413432 | 1 |
| CAGAA | 19940 | 1.9509 | 5.355729 | 1 |
| GAAGC | 22690 | 1.8683306 | 5.132398 | 4 |
| TCTGC | 21115 | 1.7889608 | 5.4758034 | 7 |
| AAAAT | 13145 | 1.7747123 | 6.8005385 | 3 |
| TTTAT | 12450 | 1.690455 | 5.964849 | 2 |
| GAAAA | 14010 | 1.5888857 | 5.2952 | 1 |
| TTATT | 11345 | 1.5404186 | 5.8711605 | 3 |
| TTGAG | 13905 | 1.3322308 | 5.002259 | 7 |
| ATCTG | 13395 | 1.3155192 | 5.4438143 | 6 |
| ATCCA | 13070 | 1.3132689 | 5.1767583 | 6 |
| AATCT | 10085 | 1.1768495 | 5.7705245 | 5 |